Dried Urine Test for Comprehensive Hormones, colloquially called the DUTCH Test, developed by Precision Analytical, is available at Toronto Centre for Naturopathic Medicine.
Hormones influence nearly every system in the body – energy, mood, metabolism, sleep, fertility and long-term disease risk.
The DUTCH Test (DUTCH Complete® and/or DUTCH Plus®) provides one of the most comprehensive and clinically validated assessments of hormone production, metabolism and your daily circadian rhythm available today.
The DUTCH Test is widely used by naturopathic doctors and integrative and functional medicine practitioners to gain detailed information that standard blood tests cannot detect, in patients suffering from fertility challenges, symptoms of perimenopause, premenstrual syndrome, low libido, anxiety or depressive symptoms, disrupted sleep, resistant weight loss and acne, among other health concerns.
At our Toronto Centre for Naturopathic Medicine, we use the DUTCH Test to uncover root causes behind complex health conditions that have proven resistant to treatment and to guide highly personalized treatment plans.
The DUTCH Test measures:
The DUTCH Complete® and DUTCH Plus® tests assess hormones and metabolites using dried urine collected over a full day. Analytes assessed include:
- Progesterone metabolites
- Estrogen and estrogen metabolites (e.g., estrone, estradiol, estriol)
- Estrogen metabolite ratios
- Androgens and androgen metabolites (e.g., testosterone, androstenedione, 5α-DHT [via metabolites], DHEA-S)
- Free cortisol and cortisone
- Cortisol metabolites
- Nutritional organic acids (e.g., markers for B12, glutathione)
- Neurological-related organic acids (e.g., markers for dopamine, epinepherine)
- Organic acids markers for melatonin and oxidative stress/DNA damage
The DUTCH Test may provide useful guidance if:
DUTCH Complete® or DUTCH Plus® may be recommended when symptoms are unexplained, persistent or hormonally driven. They may help clarify underlying causes if you experience:
- persistent fatigue or energy “crashes”
- difficulty managing stress or poor stress resilience
- sleep disruption or early morning waking
- anxiety, low mood or irritability
- weight gain resistant to diet and exercise
- irregular menstrual cycles or PMS
- perimenopausal or menopausal symptoms
- low libido or fertility challenges
- acne, hair thinning or hirsutism
- “brain fog” or poor concentration
It may also help your naturopathic doctor understand underlying causes of:
- adrenal dysregulation or chronic stress states
- estrogen dominance or impaired estrogen clearance
- polycystic ovary syndrome (PCOS)
- perimenopause and menopause symptoms
- hypothalamic-pituitary-adrenal (HPA) axis dysfunction
- androgen excess or deficiency
- mood disorders influenced by hormone metabolism
- sleep-wake circadian rhythm disruption
How does the DUTCH Test work?
DUTCH Complete® and DUTCH Plus® use dried urine samples collected at multiple points over a 24-hour period, allowing assessment of daily hormone patterns rather than single time-point values.
DUTCH Plus® adds use of saliva samples to assess your cortisol awakening response (CAR).
Precision Analytical employs gas chromatography–mass spectrometry (GC-MS) and liquid chromatography–tandem mass spectrometry (LC-MS/MS) – gold-standard laboratory techniques known for their accuracy, sensitivity and reproducibility. This methodology enables precise measurement of hormone metabolites, providing insight into how hormones are produced, broken down, and cleared, not just how much is present in blood at one moment in time.
DUTCH Test Customization
The distinction between DUTCH Complete® and DUTCH Plus® is measurement of your cortisol awakening response (CAR).
- DUTCH Complete®
- DUTCH Plus® (DUTCH Complete® “plus” measurement of cortisol awakening response)
We will help you navigate which option is appropriate for you!
Learn more about DUTCH Test (DUTCH Complete®).
Related Tests
Related tests offered at Toronto Centre for Naturopathic Medicine include:
Other tests available at Toronto Centre for Naturopathic Medicine that offer a deeper understanding of the underlying causes of persistent fatigue, difficulty concentrating, and symptoms of “being burned out” in general include:
Why work with a naturopathic doctor?
Interpreting DUTCH results requires clinical context. Hormone metabolites must be evaluated alongside symptoms, medical history, lifestyle factors and other laboratory findings.
Our naturopathic doctors use DUTCH testing to design evidence-informed, individualized treatment plans, which may include dietary therapy, targeted nutritional supplementation, herbal (phyto) medicines and lifestyle interventions.
If you are considering DUTCH testing, we invite you to book a consultation to determine whether the DUTCH Complete® or DUTCH Plus® can help you reach your health goals.
References
Precision Analytical Inc. DUTCH Test® Clinical Overview and Validation Data. Eugene, OR: Precision Analytical; accessed 2026.
Auyeung TW, Kwok T, Leung J, et al. Cortisol diurnal rhythm and its association with health outcomes. PLoS One. 2013;8(6):e67636.
Bao AM, Swaab DF. The human hypothalamus in mood disorders. Prog Brain Res. 2010;182:59-82.
Stanczyk FZ, Clarke NJ. Advantages and challenges of mass spectrometry assays for steroid hormones. J Steroid Biochem Mol Biol. 2010;121(3-5):491-495.